A few days ago, the director of Shanghai Hengyuan Technology Department Ouyang summarized the latest ELISA problem, mainly for the reasons and solutions of ELISA coloration and low sensitivity. The Shanghai Hengyuan ELISA Kit can provide full technical support. If you have any questions, you can contact the company for consultation. Let's take a look at the main content of today. Shanghai Hengyuan and you ponder over the reasons for the ELISA color:

main reason

solution

The kit is too long in transit and the temperature is too high

Try to shorten the transportation time, and cool the ice in the summer.

The kit is not fully balanced

Remove the kit from the 2 to 8 ° C refrigerator, open the lid, and equilibrate for at least 20 minutes at room temperature to ensure that all reagents are equilibrated to room temperature (approximately 25 ° C).

Incubator temperature is less than 37 ° C

Pay attention to the temperature of the incubator, and reduce the number of opening times after putting it into the reaction plate to avoid affecting the temperature. The non-isolated incubator should pay special attention to it.

Insufficient holding time

Correct the fixed clock timing

The impact force is too large during washing, the soaking time is too long, and the number of washings is increased.

Keep washing time according to the instructions, and remember the number of washings accurately

The pipette has insufficient liquid absorption, too much water is hanged on the inner wall of the nozzle or the inner wall is not clean.

Correct the pipette, the nozzle should be matched, the nozzle should be tight when it is installed, and the inner wall of the nozzle should be clean. It is best to use it once.

There is a problem with the quality of distilled water

Use fresh qualified distillation

Insufficient substrate action time

Accurate timing

ELISA shows relevant tips: color control must control the time, according to the kit instructions. In general, the color development time is too short, and the result is low; the color development time is too long, the blank is increased, or the non-specific color development is increased.

The ELISA kits supplied by Shanghai Hengyuan belong to people, rats, mice, pigs, chickens, rabbits, monkeys, cattle, plants... During the May Day period, 20% discount is also given gifts. Customers are welcome to call Shanghai Hengyuan. Biological company salesman to buy.

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