Some cell adhesion molecules are distributed on the plasma membrane of the cell, and some adhesion molecules are used as components of intercellular junctions. The function of different intercellular junction structures is different, such as desmosomes and adhesion junctions bind epithelial cells together; tight The connection can close the gap between cells (such as the secretory cells of vesicular or tubular glands, and between vascular endothelial cells); the gap connection allows ions, nutrient molecules and cAMP and other small molecular signaling substances to move between the cells in contact [ Alberts etal., 2002]. Desmosomes are also widely distributed in the plasma membrane area where the cells are in contact, but they are often co-distributed with tight junctions and adhesion junction structures near the top of the cell side.

When epithelial cells differentiate in a puffed culture, the formation of desmosomes will increase, or when a certain morphological tissue appears, various types of junction complexes will be formed. This is why the puffed culture takes too long and the epithelium The reason why cells are not easy to separate. Because many of the adhesion molecular components in these connection structures are Ca + -dependent, chelating agents such as EDTA are usually added to trypsin during or before dissociation.

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