Experimental principle of human soluble CD28 (sCD28) ELISA kit
Experimental principle of human soluble CD28 (sCD28) ELISA kit 1. The level of human soluble CD28 (sCD28) ELISAkit in the specimens was determined by double antibody sandwich method. 2. First coat the microplate with purified human soluble CD28 (sCD28) ELISAkit antibody to make a solid phase antibody. 3. Then add soluble CD28 (sCD28) ELISAkit to the microwells coated with mAb in turn, and then combine with HRP-labeled soluble CD28 (sCD28) ELISAkit antibody to form an antibody-antigen-enzyme-labeled antibody complex. After thorough washing, add Substrate TMB color development. TMB is converted into blue under the catalysis of HRP enzyme, and into the final yellow under the action of acid. 4. The color depth is positively correlated with the soluble CD28 (sCD28) ELISAkit in the sample. 5. Finally, the absorbance (OD value) is measured with a microplate reader at a wavelength of 450 nm, and the concentration of human soluble CD28 (sCD28) ELISAkit in the sample is calculated by a standard curve . Objective: To determine the content of soluble CD28 (sCD28) ELISAkit in human serum, plasma and related liquid samples. 5. Serum: The blood will naturally coagulate at room temperature for 10-20 minutes and centrifuge for about 20 minutes (2000-3000 rpm). Collect the supernatant carefully and centrifuge again if a precipitate appears during storage. Avoid any cell stimulation during the operation. Use test tubes free of pyrogens and endotoxins. 6. Plasma: EDTA or sodium citrate should be selected as the anticoagulant according to the requirements of the specimen. After mixing for 10-20 minutes, centrifuge for about 20 minutes (2000-3000 rpm) to remove particles. Collect the supernatant carefully, and centrifuge again if there is any precipitate. safety: 4 Keep Elisa kit away from children Bring your own items: 1. 37 ℃ incubator 2. Standard specification microplate reader 3. Precision pipette and disposable tip 4. Distilled water 5. Disposable test tube 6. Absorbent paper Storage conditions and validity period: 1. Kit storage :; 2-8 ℃. 2. Validity: 6 months Related Products: Human Interferon α / βReceptor, IFN-α / βR ELISA Human B cell growth protein, BCGF ELISA Human B cell differetiation factor, BCDF ELISA Human Epithelial Cell Adhesion Molecule, Ep-CAM ELISA Human soluble adhesion molecules, Sam ELISA Human Alternative macrophage activation-associated CC chemokine 1, AmAC-1 ELISA Human soluble vascular endothelial growth factor receptor-2, VEGFR-2 / sFLK-1 ELISA Human thymic stromal lymphopoietin, TSLP ELISA Human Perforin / Pore-forming protein, PF / PFP ELISA Human pleiotrophin, PTN ELISA human soluble cluster of differentiation 28, sCD28 ELISA Human lymphocyte factor ELISA Human thymus activation regulated chemokine, TARC ELISA Human Neural cell adhesion molecule ligand 1, NCAM-L1 ELISA Human Cobra venom neuronal protective factor, CVNPF ELISA Human soluble Tumor Necrosis Factorαreceptor, sTNFαR ELISA Human soluble cytokine receptor, sCKR ELISA Human soluble Factor-related Apoptosis ligand, sFASL / Apo-1 ELISA Human inhibitor of apoptosis, IAP ELISA Human colony-stimulating factor, CSF ELISA Party Tent,Fold Party Tent,Fold Up Tent,Stretch Tent Party SHANDONG CAIFENG INTERNATIONAL TRADING Co., Ltd. , https://www.zihcaifeng.com
1. Avoid direct contact with stop solution and substrate. If you accidentally come into contact with these liquids, please rinse them with water as soon as possible.
2. Do not eat, drink, smoke or use cosmetics during the experiment.
3. Do not touch any ingredients in the kit with your mouth.